Jackson|二抗715-167-003*

通過(guò)木瓜蛋白酶消化完整IgG抗體產(chǎn)生Fab片段抗體，以去除整個(gè)Fc部分，包括鉸鏈區(qū)。 這些抗體是單價(jià)的，僅含有單個(gè)抗原結(jié)合位點(diǎn)。 Fab片段的分子量為約50kDa。

基于抗原結(jié)合測(cè)定和/或ELISA，抗體與全分子小鼠IgG反應(yīng)。 它還與其他小鼠免疫球蛋白的輕鏈反應(yīng)。 未檢測(cè)到針對(duì)非免疫球蛋白血清蛋白的抗體。 抗體可以與來(lái)自其他物種的免疫球蛋白交叉反應(yīng)。

Jackson|二抗715-167-003*

Physical State: Freeze-dried solid 
Storage:

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.
Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. 
Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

Purity: The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed. 
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 
Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) 
Preservative: 0.05% Sodium Azide 

Suggested Working Concentration or Dilution Range:

1:100 - 1:800 for most applications

Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.